Ribeye antibody a-domain

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August undefined, 2024

WebbMy Research and Language Selection Sign into My Research Create My Research Account English; Help and support. Support Center Find answers to questions about products, … Webb24 feb. 2016 · In some experiments, mouse monoclonal antibodies against RIBEYE(B) domain were directly labeled with Alexa-650, as previously described (Wahl et al., 2013). Antibodies against PIP 2. A protein A-purified mouse monoclonal antibody against PIP 2 was obtained from Abcam (ab2335).

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WebbBc, The same blot as in Bb but reprobed with mouse monoclonal anti-CtBP2 antibodies which detect the B domain of RIBEYE. Similar to Bb, this blot also shows the presence of RIBEYE precipitated by the RIBEYE immune serum (lane 2) but not by the RIBEYE preimmune serum (lane 1). pune to bihar flight

IJMS Free Full-Text Critical Role of the Presynaptic Protein …

Webb10 sep. 2014 · Ribeye A-domains inconsistently localize to synaptic ribbons at 3 dpf and 5 dpf. Representative confocal maximum-intensity projection images of immunolabel or GFP in lateral line NM hair cells. http://zebrafishucl.org/killifish/ribeye-a WebbMy Research and Language Selection Sign into My Research Create My Research Account English; Help and support. Support Center Find answers to questions about products, access, use, setup, and administration.; Contact Us Have a question, idea, or some feedback? We want to hear from you. second hand containers for sale in zimbabwe

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RIBEYE, a component of synaptic ribbons: a protein

Webb10 sep. 2014 · The A-domain of RIBEYE contains three specific interaction sites that are thought to mediate RIBEYE self-association [17]. When transfected into HEK293, COS, and R28 cells, the A-domain has been shown to form discrete ribbon-like aggregates [17], [18], suggesting that the A-domain functions primarily as an aggregation domain [18]. Webb26 sep. 2008 · Antibodies—The following antibodies were used in the present study: mouse monoclonal anti-GST (Sigma), mouse monoclonal anti-MBP (New England Biolabs), anti-RIBEYE(B) domain (U2656, 12), mouse monoclonal anti-CtBP2 (BD Biosciences), and polyclonal anti-EGFP (T3743; gift of Dr. Thomas C. Südhof, Dallas, TX).Full-length bovine … second hand construction plantWebb12 maj 2010 · RIBEYE consists of a unique A domain and a B domain that is mostly identical to the protein C-terminal-binding protein 2 (CtBP2) (Schmitz et al., 2000). RIBEYE(B) domain binds nicotinamide adenine dinucleotide (NADH) with high affinity (Schmitz et al., 2000). RIBEYE(B) domain/CtBP2 is highly related to CtBP1 (for review, … pune to boisar bus

"Webb28 jan. 2024 · The newly generated mouse monoclonal RIBEYE A-domain antibody 6F4 is directed against a peptide stretch in the A-domain of RIBEYE (amino acid 83–amino acid … " - Ribeye antibody a-domain

Ribeye antibody a-domain

RIBEYE B-Domain Is Essential for RIBEYE A-Domain Stability and …

Webb(C1) RIBEYE A- from publication: Characterization of RIBEYE knock-in mice to analyze the role of RIBEYE B-domain for the assembly of the synaptic ribbon Gene Knock-in, Inner … Webb6 apr. 2024 · Anti-RIBEYE(B): Protein A-purified mouse monoclonal antibody against RIBEYE(B) (clone 2D9 44) was used in a 1:300 dilution (final antibody concentration of ≈ …

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Webb3 jan. 2024 · Das Protein Ribeye kann unterteilt werden in eine für Ribeyespezifische aminoterminale A-Domäne, die primär strukturelle Aufgaben besitzt, und eine … Webb1 dec. 2000 · RIBEYE is composed of a unique A domain specific for ribbons, and a B domain identical with CtBP2, a transcriptional repressor that in turn is related to 2 …

WebbRibeye is a self-aggregating protein and is one of the major scaffolding components of the ribbon on which the neurotransmitter containing vesicles are tethered. The protein … Webb20 mars 2024 · RIBEYE is a main protein component of synaptic ribbons and is necessary to build the synaptic ribbon. RIBEYE consists of a unique A-domain and a …

Webb8 dec. 2024 · Ribeye-A protein is a scaffolding component in the ribbon where vesicles are tethered, its antibody has been found to label synapses (Shankhwar et al., 2024). Many … WebbSchematic of epitopes used to create antibodies against the AZ proteins used in this study. Antibodies designed against CAST and RIM2 were based on protein amino acid (a.a.) ... ribeye B-domain (ctbp-2) mouse ribeye-A-domain 940-1107 aa 1 1572aa N- -C 656-670 aa 1 957 aa N- -C 182-408 aa 1 ~1700 aa N- -C mouse bassoon mouse Ca v 1.4 mouse* …

Webb14 apr. 2024 · Postsynaptic target neurons of the photoreceptor ribbon synapse (bipolar cells and horizontal cells) were immunolabeled with anti-PKC antibody and anti-calbindin antibody, respectively. The thickness of each layer was not significantly different between control and CAST/ELKS Crx-Cre double cKO mice.

Webb28 jan. 2024 · Synaptic ribbons are largely composed of RIBEYE, a protein containing an N-terminal A-domain and a carboxyterminal B-domain that is identical with CtBP2, a … second hand cookers belfastWebb20 maj 2024 · RIBEYE and CtBP2 are transcribed from the same gene, with CtBP2 showing wide expression in many tissues and RIBEYE expression restricted to a few tissues. The unique ability of RIBEYE to form... pune to blr flightsWebbAnti-RIBEYE B-domain and anti-synaptophysin antibodies were applied to dissociated rod bipolar cells from both wild-type and mGluR6-deficient mice for confocal … pune to blr trainWebb(D1,D2) Retinal lysate from RBE WT/KO and RBE KO/KO mice were probed by WB analyses with anti-RIBEYE A-domain antibody 6F4 (D1) and anti-actin (D2). Anti-actin signals were used as a loading control. pune to bhuntar flightsWebbRibeye is a self-aggregating protein and is one of the major scaffolding components of the ribbon on which the neurotransmitter containing vesicles are tethered. The protein … pune to bokaro flightWebbDas Protein Ribeye kann unterteilt werden in eine für Ribeyespezifische aminoterminale A-Domäne, die primär strukturelle Aufgaben besitzt, und eine carboxyterminale B-Domäne, welche zu großen... pune to bhubaneswar flight air asiaWebbWe have partially purified synaptic ribbons from retina and identified a major protein component called RIBEYE. RIBEYE is composed of a unique A domain specific for … second hand cooker for sale